â²uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructions
نویسندگان
چکیده
منابع مشابه
Integrons and the Origin of Antibiotic Resistance Gene Cassettes
I ntegrons, which are natural genetic engineering platforms, can incorporate open reading frames and convert them to functional genes by ensuring correct expression. Although such integrons play a major role in multidrug resistance phenomena among gram-negative species, similar platforms occur in numerous bacterial species, where they can play other roles. Meanwhile, we have evidence suggesting...
متن کاملRAC: Repository of Antibiotic resistance Cassettes
Antibiotic resistance in bacteria is often due to acquisition of resistance genes associated with different mobile genetic elements. In Gram-negative bacteria, many resistance genes are found as part of small mobile genetic elements called gene cassettes, generally found integrated into larger elements called integrons. Integrons carrying antibiotic resistance gene cassettes are often associate...
متن کاملHygromycin B and Apramycin Antibiotic Resistance Cassettes for Use in Campylobacter jejuni
Campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. In this study, two antibiotic resistance cassettes were developed, encoding for hygromycin B and apramycin resistance, for use in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. First, the mark...
متن کاملGene cassettes and cassette arrays in mobile resistance integrons.
Gene cassettes are small mobile elements, consisting of little more than a single gene and recombination site, which are captured by larger elements called integrons. Several cassettes may be inserted into the same integron forming a tandem array. The discovery of integrons in the chromosome of many species has led to the identification of thousands of gene cassettes, mostly of unknown function...
متن کاملConstruction of targeted insertion mutations in Francisella tularensis subsp. novicida.
Francisella tularensis is one of the most deadly bacterial agents, yet most of the genetic determinants of pathogenesis are still unknown. We have developed an efficient targeted mutagenesis strategy in the model organism F. tularensis subsp. novicida by utilizing universal priming of optimized antibiotic resistance cassettes and splicing by overlap extension (SOE). This process enables fast an...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1992
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1992.tb05105.x